Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19498] to Trypsin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Trypsin antibody [EPR19498] - BSA and Azide free
    See all Trypsin primary antibodies

  • Description

    Rabbit monoclonal [EPR19498] to Trypsin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This product reacts with human Trypsin 1, Trypsin 3 and Putative trypsin 6 but not with Trypsin 2. However, it might cross react with other family members and isoforms.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251326 is the carrier-free version of ab200997. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251326 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human PRSS2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27, 33 kDa



    This data was developed using ab200997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2: 3 minutes.

    Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/1000 dilution

    Lane 1 : Human PRSS3 full length recombinant protein
    Lane 2 : Human PRSS3P2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27, 33 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab200997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Western blot - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/2000 dilution

    Lane 1 : Human pancreas tissue lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human fetal heart tissue lysate
    Lane 4 : Human fetal kidney tissue lysate
    Lane 5 : Human fetal spleen tissue lysate
    Lane 6 : Human fetal lung tissue lysate
    Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
    Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27, 33 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab200997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice (PMID: 14726524, 3011602, 6698368, and 9099703). This product recognizes PRSS1, and shows some cross-reactivity with PRSS3 and PRSS3P2 proteins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    This data was developed using ab200997, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells of Human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    This data was developed using ab200997, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    This data was developed using ab200997, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    This data was developed using ab200997, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)
    Anti-Trypsin antibody [EPR19498] - BSA and Azide free (ab251326)

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