Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRIM5 alpha antibody (ab109709)

Key features and details

  • Rabbit polyclonal to TRIM5 alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TRIM5 alpha antibody
    See all TRIM5 alpha primary antibodies

  • Description

    Rabbit polyclonal to TRIM5 alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Placenta; Human Thymus; Human Tonsil.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Chimpanzee
Macaque monkey
Gorilla
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.

  • Domain

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.

  • Post-translational
    modifications

    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).

  • Cellular localization

    Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt

  • Database links

    • Alternative names

      • RING finger protein 88 antibody
      • RNF88 antibody
      • TRIM5 antibody
      • TRIM5_HUMAN antibody
      • TRIM5alpha antibody
      • Tripartite motif containing 5 antibody
      • tripartite motif protein TRIM5 antibody
      • Tripartite motif-containing protein 5 antibody
      see all

    Images

    • Western blot - Anti-TRIM5 alpha antibody (ab109709)
      Western blot - Anti-TRIM5 alpha antibody (ab109709)
      All lanes : Anti-TRIM5 alpha antibody (ab109709) at 1 µg/ml

      Lane 1 : Human placenta tissue lysate - total protein (ab29745)
      Lane 2 : Tonsil (Human) Tissue Lysate
      Lane 3 : Human thymus tissue lysate - total protein (ab30146)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 56 kDa
      Additional bands at: 31 kDa (possible isoform), 46 kDa (possible isoform)


      Exposure time: 2 minutes


      This antibody was raised against an immunogen that is predicted to recognize isoforms Alpha, Beta and Epsilon of TRIM5. The predicted molecular weights of isoforms Alpha, Beta and Epsilon are 56, 46 and 31 kDa respectively.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab109709)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab109709)
      IHC image of TRIM5 alpha staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109709, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (0)

    Publishing research using ab109709? Please let us know so that we can cite the reference in this datasheet.

    ab109709 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-TRIM5 alpha antibody (ab109709)
      Western blot - Anti-TRIM5 alpha antibody (ab109709)
      All lanes : Anti-TRIM5 alpha antibody (ab109709) at 1 µg/ml

      Lane 1 : Human placenta tissue lysate - total protein (ab29745)
      Lane 2 : Tonsil (Human) Tissue Lysate
      Lane 3 : Human thymus tissue lysate - total protein (ab30146)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 56 kDa
      Additional bands at: 31 kDa (possible isoform), 46 kDa (possible isoform)


      Exposure time: 2 minutes


      This antibody was raised against an immunogen that is predicted to recognize isoforms Alpha, Beta and Epsilon of TRIM5. The predicted molecular weights of isoforms Alpha, Beta and Epsilon are 56, 46 and 31 kDa respectively.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab109709)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab109709)
      IHC image of TRIM5 alpha staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109709, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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