Anti-TRIM5 alpha antibody (ab4389)
Key features and details
- Goat polyclonal to TRIM5 alpha
- Suitable for: WB, IHC-P
- Reacts with: Cat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM5 alpha antibody
See all TRIM5 alpha primary antibodies -
Description
Goat polyclonal to TRIM5 alpha -
Host species
Goat -
Specificity
This antibody is expected to recognise all three human isoforms. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
ASGILVNVKEEVTC
, corresponding to N terminal amino acids 2-15 of Human RNF88. -
General notes
Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
Predicted band size: 38 kDa
Observed band size: 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
A) positive cells next to a secondary follicle
B) positive cells below tonsillar epithelium.
In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.
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Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
