Anti-ADAM22 antibody (ab227546)
Key features and details
- Rabbit polyclonal to ADAM22
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM22 antibody
See all ADAM22 primary antibodies -
Description
Rabbit polyclonal to ADAM22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Positive control
- WB: Human brain tissue extract, U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-Fr: Embryonic mouse brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ADAM22 antibody (ab227546) at 1/500 dilution
Lane 1 : U87-MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody at 1/10000 dilution
Predicted band size: 100 kDa10% SDS gel
Running condition: 80V, 15min; 140V, 40 min
Primary antibody incubation: 4C overnight
Washing condition: 5 mL TBST, 4 x 5 minutes -
Anti-ADAM22 antibody (ab227546) at 1/500 dilution + Human brain tissue extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 100 kDa
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Frozen sectioned embryonic mouse brain (mE18.5) stained for ADAM22 with ab227546 (1/500 dilution) (red) in immunohistochemical analysis. Counterstains: DAPI (blue).