Human ADAMTS13 ELISA Kit (ab234559)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 0.125 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ADAMTS13 ELISA Kit
See all ADAMTS13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.4% Inter-assay Sample n Mean SD CV% Serum 3 2.6% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
0.125 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109 105% - 115% Tissue Extracts 96 93% - 100% Cell culture media 99 97% - 100% Hep Plasma 107 103% - 111% EDTA Plasma 100 98% - 104% Cit plasma 102 100% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human ADAMTS13 ELISA Kit (ab234559) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ADAMTS13 protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ADAMTS13 with 16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human ADAMTS13 protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.
Urine, milk, and saliva samples have not been tested with this kit.
INTERFERENCE
Recombinant human Von Willebrand Factor was prepared at 50 ng/mL and 1 ng/mL and tested for interference with 5 ng/mL recombinant human ADAMTS13. Interference of 9% was observed with 50 ng/mL Von Willebrand Factor. No interference with was observed 1 ng/mL Von Willebrand Factor.
SPECIES REACTIVITY
This kit recognizes human ADAMTS13 protein.
Other species reactivity was determined by measuring 1:200 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ADAMTS13 Capture Antibody 1 x 600µl 10X Human ADAMTS13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human ADAMTS13 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cleaves the vWF multimers in plasma into smaller forms. -
Tissue specificity
Plasma. Expressed primarily in liver. -
Involvement in disease
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. -
Sequence similarities
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains. -
Domain
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction. -
Post-translational
modificationsMay contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 13
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 13
see all -
Database links
- Entrez Gene: 11093 Human
- Omim: 604134 Human
- SwissProt: Q76LX8 Human
- Unigene: 131433 Human
Images
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Other - Human ADAMTS13 ELISA Kit (ab234559)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ADAMTS13 standard curve in Sample Diluent NS.The ADAMTS13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Human ASAMTS13 Competitor Std Curve ComparisonStandard curve comparison between human ADAMTS13 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human ADAMTS13 standard curve in Sample Diluent NS.The ADAMTS13 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human ADAMTS13 standard curve in 1X Cell Extraction Buffer PTR.The ADAMTS13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human ADAMTS13 standard curve in 1X Cell Extraction Buffer PTR.The ADAMTS13 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ADAMTS13 in human serum and plasma samples.The concentrations of ADAMTS13 were measured in duplicates, interpolated from the ADAMTS13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (heparin) 1:200 and plasma (EDTA) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 1,082 ng/mL in neat serum, 963.3 ng/mL in neat plasma (citrate), 760.3 ng/mL in neat plasma (heparin), and 681.5 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native ADAMTS13 in human liver tissue extract based on a 1,000 µg/mL extract load.The concentrations of ADAMTS13 were measured in duplicate and interpolated from the ADAMTS13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 0.364 ng/mL in human liver tissue extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAMTS13 concentration was determined to be 990.8 ng/mL with a range of 687.3 – 1,346 ng/mL.
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Linearity of dilution in Sample Diluent NS.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native ADAMTS13 was measured in serum and plasma samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant ADAMTS13 was spiked into neat RPMI cell culture media containing 10% FBS and diluted in a 2-fold dilution series in Sample Diluent NS.
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Linearity of dilution in 1X Cell Extraction Buffer PTR.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native ADAMTS13 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Sandwich ELISA - Human ADAMTS13 ELISA Kit (ab234559)To learn more about the advantages of recombinant antibodies see here.
