Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TRIB2 antibody [OTI8D11] (ab117981)

Key features and details

Mouse monoclonal [OTI8D11] to TRIB2
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Dog, Human, African green monkey
Isotype: IgG2b

Product name

Anti-TRIB2 antibody [OTI8D11]
See all TRIB2 primary antibodies
Description

Mouse monoclonal [OTI8D11] to TRIB2
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human TRIB2 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_067675).
Database link: Q92519

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY TRIB2 cDNA; MCF7 cell extract. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney carcinoma, ovary adenocarcinoma, thyroid carcinoma, endometrium and bladder carcinoma tissues. ICC/IF: COS7 cells. FLow Cyt: HEK-293T cells transfected with TRIB2 overexpress plasmid.
General notes

Clone OTI8D11 (formerly 8D11).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI8D11
Isotype

IgG2b
Research areas

Western blot - Anti-TRIB2 antibody [OTI8D11] (ab117981)

All lanes : Anti-TRIB2 antibody [OTI8D11] (ab117981) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY TRIB2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 39 kDa
Western blot - Anti-TRIB2 antibody [OTI8D11] (ab117981)

All lanes : Anti-TRIB2 antibody [OTI8D11] (ab117981) at 1/1000 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 39 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201210)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human breast adenocarcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human colon adenocarcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human kidney carcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human ovary adenocarcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human thryoid carcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human endometrium tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Paraffin-embedded human bladder carcinoma tissue stained for TRIB2 using ab117981 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-TRIB2 antibody [OTI8D11] (ab117981)

COS-7 (african green monkey kidney fibroblast-like cell line) cells stained for TRIB2 (green) using ab117981 at 1/50 dilution in ICC/IF.
Flow Cytometry - Anti-TRIB2 antibody [OTI8D11] (ab117981)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either TRIB2 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling TRIB2 with ab117981 at 1/50 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRIB2 antibody [OTI8D11] (ab117981)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Images

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