Anti-Transferrin Receptor antibody [R17 217.1.3] - Chimeric (ab281913)
![Anti-Transferrin Receptor antibody [R17 217.1.3] - Chimeric (ab281913)](https://www.abcam.com/ps/products/281/ab281913/Images/ab281913-459056-AntiTransferrin-Receptor-antibody-immuhistochemistry-RAW-2647-cells-.jpg "Anti-Transferrin Receptor antibody [R17 217.1.3] - Chimeric (ab281913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [R17 217.1.3] to Transferrin Receptor - Chimeric
- Suitable for: Flow Cyt, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-Transferrin Receptor antibody [R17 217.1.3] - Chimeric
See all Transferrin Receptor primary antibodies -
Description
Rabbit monoclonal [R17 217.1.3] to Transferrin Receptor - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Transferrin Receptor. Fusion of murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
Database link: Q62351 -
Positive control
- ICC:RAW 264.7 cells. Flow cyt: RAW 264.7 cells.
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General notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R17 217.1.3 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [R17 217.1.3] (ab281913)
Immunofluorescence analysis of paraformaldehyde fixedRAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling Transferrin Receptor with ab281913 at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/ tpo-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.
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![Flow Cytometry - Anti-Transferrin Receptor antibody [R17 217.1.3] (ab281913)](https://www.abcam.com/ps/products/281/ab281913/Images/ab281913-1-AntiTransferrin-Receptor-antibody-flowcytometry-RAW-2647-cells.jpg)
Flow Cytometry - Anti-Transferrin Receptor antibody [R17 217.1.3] (ab281913)Flow-cytometry analysis of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling Transferrin Receptor with anti-Fluorescein IgG antibody (isotype control, black line) or ab281913 (blue line) at 1/100 dilution for 1 hour at room temperature. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at 1/1000 dilution and cells analysed using a FACSCanto flow-cytometer.
