Human Fetuin A ELISA Kit (AHSG) (ab108855)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fetuin A ELISA Kit (AHSG)
See all AHSG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
> 3 ng/ml -
Range
3.13 ng/ml - 200 ng/ml -
Recovery
= 99 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.
A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.
Get higher sensitivity in only 90 minutes with Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Fetuin A Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Fetuin A Microplate (12 x 8 well strips) 1 unit Fetuin A Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
Tissue specificity
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 59 kDa bone sialic acid-containing protein
- A2HS
- Aa2-066
see all -
Database links
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108855.