Immunohistochemistry analysis of STNFR1 showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 minutes at room temperature, washed with ddH₂O and PBS, and then probed with a STNFR1 Recombinant Rabbit Multiclonal Antibody (ab277793) diluted in 3% BSA-PBS at a dilution of 1/20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.