Human DNA PKcs peptide (ab20407)
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Product name
Human DNA PKcs peptide
See all DNA PKcs proteins and peptides -
Animal free
No -
Nature
Synthetic -
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Species
Human
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Images
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Western blot - Human DNA PKcs peptide (ab20407)All lanes : Anti-DNA PKcs (phospho S2056) antibody (ab18192) at 1 µg/ml
Lane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg
Lane 2 : 20ug of untreated HeLa cell extract
Lane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
Lane 4 : 20ug of untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
Lane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg withHuman DNA PKcs peptide (ab20407) at 1 µg/ml
Lane 6 : 20ug of untreated HeLa cell extract withHuman DNA PKcs peptide (ab20407) at 1 µg/ml
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700)
at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 460 kDa why is the actual band size different from the predicted?
Additional bands at: 270 kDa (possible cleavage fragment), 270 kDa (possible cross reactivity)ab18192 specifically recognizes a band at ~460 kDa corresponding to DNA PKcs in HeLa cells that have been treated with ionizing radiation (lane 1). This band is not detected in untreated cells (lane 2). The activity of the antibody is quenched by the addition of the immunizing (modified) peptide, ab20406 (lanes 3) but not the unmodified peptide, ab20407 (lane 5).
For the ab13823 irradiated HeLa cell lysate, the 4 hour post-treatment extract was used.
