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Anti-TMEM161A antibody [EPR14369] (ab180954)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-TMEM161A antibody [EPR14369] (ab180954)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14369] to TMEM161A
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-TMEM161A antibody [EPR14369]
    See all TMEM161A primary antibodies
  • Description

    Rabbit monoclonal [EPR14369] to TMEM161A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT 1080, HepG2 and Human fetal liver lysates; Human lung adenocarcinoma tissue; Human infiltrating duct carcinoma of breast tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14369
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Receptor Processing

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] (ab180954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] (ab180954)

    Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] (ab180954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] (ab180954)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TMEM161A antibody [EPR14369] (ab180954)
    Western blot - Anti-TMEM161A antibody [EPR14369] (ab180954)
    Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-TMEM161A antibody [EPR14369] (ab180954)
    Western blot - Anti-TMEM161A antibody [EPR14369] (ab180954)
    All lanes : Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/100000 dilution

    Lane 1 : HT1080 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?

  • Anti-TMEM161A antibody [EPR14369] (ab180954)
    Anti-TMEM161A antibody [EPR14369] (ab180954)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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