Anti-Nuclei antibody [235-1] (ab254080)
![Anti-Nuclei antibody [235-1] (ab254080)](https://www.abcam.com/ps/products/254/ab254080/Images/ab254080-344059-antinucleiantibody2351-immunocytochemistryimmunofluorescence-hela-human.jpg "Anti-Nuclei antibody [235-1] (ab254080)")
Key features and details
- Mouse monoclonal [235-1] to Nuclei
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nuclei antibody [235-1] -
Description
Mouse monoclonal [235-1] to Nuclei -
Host species
Mouse -
Specificity
Specific for endogenous levels of human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band.
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Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Nuclei. Human cell homogenate.
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Positive control
- ICC/IF: HeLa cells.
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General notes
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
235-1 -
Isotype
IgG1
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nuclei antibody [235-1] (ab254080)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nuclei using ab254080 at 1/100 dilution in ICC/IF (green).