ICC/IF image of ab3581 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3581, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Glucocorticoid Receptor beta antibody (ab3581) at 1/500 dilution

Lane 1 : COS-74 whole cell extract transfected with Glucocorticoid Receptor alpha
Lane 2 : COS-74 whole cell extract transfected with Glucocorticoid Receptor beta

Predicted band size: 83 kDa