Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
![Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-211159-anti-nsd3-antibody-epr13813-n-terminal-western-blot.jpg "Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13813] to NSD3 - N-terminal
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NSD3 antibody [EPR13813] - N-terminal
See all NSD3 primary antibodies -
Description
Rabbit monoclonal [EPR13813] to NSD3 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human NSD3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9BZ95 -
Positive control
- Hela, A673 and MCF7 lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13813 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (unpurified)
Lane 1 : Hela lysate
Lane 2 : A673 lysate
Lane 3 : MCF7 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 162 kDa
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![Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-241059-anti-nsd3-antibody-epr13813-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab180500 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of primary and secondary antibody were used as a negative control (blue line).
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-241058-anti-nsd3-antibody-epr13813-n-terminal-western-blot.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 162 kDa
Observed band size: 162 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-241057-anti-nsd3-antibody-epr13813-n-terminal-western-blot.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 162 kDa
Observed band size: 162 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-241056-anti-nsd3-antibody-epr13813-n-terminal-western-blot.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 162 kDa
Observed band size: 162 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-211160-anti-nsd3-antibody-epr13813-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Flow Cytometrical analysis of HeLa cells labeling NSD3 with unpurified ab180500 at 1/75 (green) compared to a negative control antibody (red).
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![Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](https://www.abcam.com/ps/products/180/ab180500/Images/ab180500-8-benefits-of-recombinant-antibodies.png)
Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
