Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23943-149] to TIMP3 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free
    See all TIMP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23943-149] to TIMP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231 whole cell lysate; Human lung and placenta tissue lysates; Recombinant Human TIMP-3 Protein (aa24-211). Flow cyt: MDA-MB-231 cells. ICC: MDA-MB-231 cells.

  • General notes

    ab276139 is the carrier-free version of ab276134. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab276139 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Immunocytochemistry - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)

    This data was developed using ab276134, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling TIMP3 with ab276134 at 1/50 (9.16 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-231 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    All lanes : Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 24 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab276134, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Low expression tissue: human liver (PMID:8174111).

    Exposure time: 125 seconds.

  • Flow Cytometry - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Flow Cytometry - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)

    This data was developed using ab276134, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labelling TIMP3 with ab276134 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Recombinant Human TIMP-3 Protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab276134, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: 1 second.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + Human lung tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 24 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    This data was developed using ab276134, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Exposure time: 92 seconds.

  • Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Western blot - Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Anti-TIMP3 antibody [EPR23943-149] (ab276134) at 1/1000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    This data was developed using ab276134, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8174111, 30894640).

    Exposure time: 3 minutes.

  • Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)
    Anti-TIMP3 antibody [EPR23943-149] - BSA and Azide free (ab276139)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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