Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)
![Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)](https://www.abcam.com/ps/products/248/ab248295/Images/ab248295-402372-anti-hmbs-pbgd-antibody-epr8105-bsa-and-azide-free-western-blot.jpg "Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8105] to HMBS/PBGD - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free
See all HMBS/PBGD primary antibodies -
Description
Rabbit monoclonal [EPR8105] to HMBS/PBGD - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248295 is the carrier-free version of ab129092 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as HMBS
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.94 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8105 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)All lanes : Anti-HMBS/PBGD antibody [EPR8105] (ab129092) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : U937 cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 42-44 kDa why is the actual band size different from the predicted?This data was developed using ab129092, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)](https://www.abcam.com/ps/products/248/ab248295/Images/ab248295-1-anti-hmbs-pbgd-antibody-epr8105-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)This data was developed using ab129092, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)](https://www.abcam.com/ps/products/248/ab248295/Images/ab248295-3-benefits-of-recombinant-antibodies.png)
Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free (ab248295)