Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)
![Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)](https://www.abcam.com/ps/products/243/ab243903/Images/ab243903-334405-anti-tigit-antibody-blr047f-immunohistochemistry.jpg "Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR047F] to TIGIT - BSA free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TIGIT antibody [BLR047F] - BSA free
See all TIGIT primary antibodies -
Description
Rabbit monoclonal [BLR047F] to TIGIT - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human TIGIT aa 194-244 (C terminal). The exact sequence is proprietary. NP_776160.2 and Gene ID 201633.
Database link: Q495A1 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR047F -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Western blot - Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)](https://www.abcam.com/ps/products/243/ab243903/Images/ab243903-334406-anti-tigit-antibody-blr047f-western-blot.jpg)
Western blot - Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)Western blot analysis using ab243903 at 1/1000 dilution.
Lane 1: HeLa cell whole cell lysate (50 µg).
Lane 2: HEK-293T cell whole cell lysate (50 µg).
Lane 3: MyLa CD4+ whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: MG whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: GaMG whole cell lysate (50 µg).
Lane 8: A549 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes. -
![Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)](https://www.abcam.com/ps/products/243/ab243903/Images/ab243903-3-benefits-of-recombinant-antibodies.png)
Anti-TIGIT antibody [BLR047F] - BSA free (ab243903)
