Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
![Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.com/ps/products/126/ab126622/Images/ab126622-140617-anti-edg3-s1p3-antibody-epr45412-western-blot.jpg "Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4541(2)] to EDG3/S1P3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EDG3/S1P3 antibody [EPR4541(2)]
See all EDG3/S1P3 primary antibodies -
Description
Rabbit monoclonal [EPR4541(2)] to EDG3/S1P3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human EDG3/S1P3 aa 300-150.
Database link: Q99500 -
Positive control
- Human kidney tissue; Human heart, HepG2 and 293T cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as EDG3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4541(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)All lanes : Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human heart tissue lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.com/ps/products/126/ab126622/Images/ab126622-140616-anti-edg3-s1p3-antibody-epr45412-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)ab126622, at a 1/50 dilution, staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.com/ps/products/126/ab126622/Images/ab126622-140615-anti-edg3-s1p3-antibody-epr45412.jpg)
OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) -
![Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)](https://www.abcam.com/ps/products/126/ab126622/Images/ab126622-4-benefits-of-recombinant-antibodies.png)
Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622)
