Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10308] to TG6 - N-terminal
  • Suitable for: WB, ICC, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TG6 antibody [EPR10308] - N-terminal
    See all TG6 primary antibodies

  • Description

    Rabbit monoclonal [EPR10308] to TG6 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Mouse
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MOLT-4, CTLL-2, EL4, RAW 264.7 and NIH/3T3 cell lysates; Human kidney and fetal kidney lysates; Mouse kidney lysate. ICC/IF: and MOLT-4, RAW 264.7 cells. IHC-P: Human adenocarcinoma of endometrium tissue; Flow Cyt: MOLT-4 cells; ICC: MOLT-4 and Raw 264.7 cells.

  • General notes

     

     

    Previously labelled as Transglutaminase 6. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Immunocytochemistry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing membranous staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

    Immunohistochemical analysis of paraffin- embedded Human adenocarcinoma of endometrium tissue labeling TG6 with ab180959 at 1/100 dilution. Secondary antibody ImmunoHistoprobe HRP Polymer for Rabbit IgG pre-diluted.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    All lanes : Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/1000 dilution

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : CTLL-2 (Mouse T lymphocyte) cell lysate
    Lane 5 : EL4 (Mouse lymphoma T lymphocyte) cell lysate
    Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 70 s

  • Flow Cytometry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Flow Cytometry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

    Flow cytometric analysis of 2% paraformaldehyde fixed MOLT4 cells labelling TG6 (green) or negative control (red) using ab180959 at 1/20 dlution followed by Goat anti rabbit IgG (FITC) at 1/150 diltuion.

  • Immunocytochemistry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Immunocytochemistry - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing membranous staining in MOLT-4 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

  • Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/5000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa

  • Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Western blot - Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/2000 dilution + MOLT-4 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

  • Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)
    Anti-TG6 antibody [EPR10308] - N-terminal (ab180959)

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