Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10308] to TG6 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TG6 antibody [EPR10308] - BSA and Azide free
    See all TG6 primary antibodies

  • Description

    Rabbit monoclonal [EPR10308] to TG6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: Raw 264.7 and MOLT-4 cells. WB: MOLT-4, CTLL-2, EL4, RAW 264.7 and NIH/3T3 cell lysates; Human kidney lysate; Mouse kidney lysate.

  • General notes

    Ab250287 is the carrier-free version of ab180959. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250287 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)
    Immunocytochemistry - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing membranous staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180959).

  • Western blot - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)
    Western blot - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)
    All lanes : Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/1000 dilution

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : CTLL-2 (Mouse T lymphocyte) cell lysate
    Lane 5 : EL4 (Mouse lymphoma T lymphocyte) cell lysate
    Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     

     Exposure time: 70 s

  • Immunocytochemistry - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)
    Immunocytochemistry - Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling TG6 with ab180959 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing membranous staining in MOLT-4 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180959).

  • Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)
    Anti-TG6 antibody [EPR10308] - BSA and Azide free (ab250287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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