Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 20 µl 110 563 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR070G] to TFEB
Suitable for: IP, WB
Reacts with: Mouse, Human

Product name

Anti-TFEB antibody [BLR070G]
See all TFEB primary antibodies
Description

Rabbit monoclonal [BLR070G] to TFEB
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human TFEB aa 426-476 (C terminal). The exact sequence is proprietary. NP_009093.1.
Database link: P19484
Positive control
- WB: HeLa, A549, HepG2, Jurkat, RKO, K562, LNcaP, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 0.6% Boric Acid, 0.9% Sodium borate, 0.4% Sodium chloride
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR070G
Isotype

IgG
Research areas

Western blot - Anti-TFEB antibody [BLR070G] (ab264421)

All lanes : Anti-TFEB antibody [BLR070G] (ab264421) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : RKO whole cell lysate
Lane 6 : K562 whole cell lysate
Lane 7 : LNcaP whole cell lysate
Lane 8 : MCF7 whole cell lysate
Lane 9 : TCMK-1 whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 53 kDa

Exposure time: 3 minutes

Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-TFEB antibody [BLR070G] (ab264421)

TFEB was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264421 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264421 at 1/1000 dilution.

Lane 1: ab264421 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.

Detection: Chemiluminescence with an exposure time of 75 seconds.

Lysates prepared using NETN lysis buffer.
Anti-TFEB antibody [BLR070G] (ab264421)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com