Anti-TFEB antibody (ab2636)
Key features and details
- Goat polyclonal to TFEB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFEB antibody
See all TFEB primary antibodies -
Description
Goat polyclonal to TFEB -
Host species
Goat -
Specificity
This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
Sequence:ASRIGLRMQLMRE
Database link: P19484 -
Positive control
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General notes
Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody
Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TFEB antibody (ab2636)Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysatePrimary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-TFEB antibody (ab2636)Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
