Anti-TFEB antibody (ab245349)
Key features and details
- Rabbit polyclonal to TFEB
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFEB antibody
See all TFEB primary antibodies -
Description
Rabbit polyclonal to TFEB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human TFEB aa 325-375. The exact sequence is proprietary. NP_009093.1
Database link: P19484 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245349 was affinity purified using an epitope specific to TFEB immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TFEB antibody (ab245349)All lanes : Anti-TFEB antibody (ab245349) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 10 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-TFEB antibody (ab245349)TFEB was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245349 used for IP at 6 µg per reaction. For WB 1 µg/ml.Lane 1: ab245349 (Lot 2) IP in HeLa whole cell lysate.
Lane 2: ab245349 (Lot 3) IP in HeLa whole cell lysate.
Lane 3: Control IgG.
Chemiluminescence detection: 10 seconds.
