Anti-MTR antibody (ab66039)
Key features and details
- Rabbit polyclonal to MTR
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MTR antibody
See all MTR primary antibodies -
Description
Rabbit polyclonal to MTR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Pig, Caenorhabditis elegans, Chimpanzee
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human MTR.
Read Abcam's proprietary immunogen policy (Peptide available as ab69085.) -
Positive control
- This antibody gave a positive signal in the following lysates: TE 671 Whole Cell, Mouse Liver Tissue, Rat Thymus Tissue, Mouse Pancreas Tissue; Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Bleomycin Treated (20U/ml), HeLa Whole Cell, Mouse Kidney Tissue, Rat Liver Tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab66039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Relevance
MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 4548 Human
- Entrez Gene: 238505 Mouse
- Entrez Gene: 81522 Rat
- NCBI: 4557765 Human
- SwissProt: Q99707 Human
- SwissProt: A6H5Y3 Mouse
- SwissProt: Q9Z2Q4 Rat
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Alternative names
- 5-methyltetrahydrofolate homocysteine methyltransferase antibody
- 5-methyltetrahydrofolate-homocysteine methyltransferase antibody
- cblG antibody
see all
Images
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Western blot - Anti-MTR antibody (ab66039)All lanes : Anti-MTR antibody (ab66039) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 7 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : Kidney (Mouse) Tissue Lysate
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa
Additional bands at: 117 kDa, 35 kDa, 50 kDa, 55 kDa, 65 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-MTR antibody (ab66039)ICC/IF image of ab66039 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66039, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR antibody (ab66039)IHC image of ab66039 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (6)
ab66039 has been referenced in 6 publications.
- Lei L et al. Attenuated expression of MTR in both prenatally androgenized mice and women with the hyperandrogenic phenotype of PCOS. PLoS One 12:e0187427 (2017). PubMed: 29232372
- Bito T et al. A dodecylamine derivative of cyanocobalamin potently inhibits the activities of cobalamin-dependent methylmalonyl-CoA mutase and methionine synthase of Caenorhabditis elegans. FEBS Open Bio 4:722-9 (2014). WB ; Caenorhabditis elegans . PubMed: 25161880
- Pérez-Sepúlveda A et al. Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia. Biomed Res Int 2013:731962 (2013). WB ; Human . PubMed: 24024209
- Cherukad J et al. Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development. Placenta 33:440-8 (2012). PubMed: 22365888
- Sáez-Ayala M et al. Melanoma coordinates general and cell-specific mechanisms to promote methotrexate resistance. Exp Cell Res 318:1146-59 (2012). PubMed: 22484375
- Murakami M et al. Improving drug potency and efficacy by nanocarrier-mediated subcellular targeting. Sci Transl Med 3:64ra2 (2011). PubMed: 21209412
Images
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Western blot - Anti-MTR antibody (ab66039)All lanes : Anti-MTR antibody (ab66039) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 7 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : Kidney (Mouse) Tissue Lysate
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa
Additional bands at: 117 kDa, 35 kDa, 50 kDa, 55 kDa, 65 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
-
Immunocytochemistry/ Immunofluorescence - Anti-MTR antibody (ab66039)ICC/IF image of ab66039 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66039, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR antibody (ab66039)IHC image of ab66039 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
