Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)
![Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)](https://www.abcam.com/ps/products/249/ab249797/Images/ab249797-402948-anti-tapp-1-antibody-epr12061-bsa-and-azide-free-western-blot.jpg "Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12061] to TAPP-1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free
See all TAPP-1 primary antibodies -
Description
Rabbit monoclonal [EPR12061] to TAPP-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249797 is the carrier-free version of ab174292. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249797 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12061 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)All lanes : Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/1000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Human fetal thymus cell lysate
Lane 6 : Human skeletal muscle cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDaThis data was developed using ab174292, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)](https://www.abcam.com/ps/products/249/ab249797/Images/ab249797-399696-anti-tapp-1-antibody-epr12061-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)This data was developed using ab174292, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from (1) fetal lung lysate or (2) 1XPBS (negative control) using ab172627, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Lane 1 Primary: ab172627, 1/1000 dilution. Sample: Fetal lung lysate.
Lane 2 Primary: ab172627, 1/1000 dilution. Sample: 1X PBS (negative control).
Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/10 dilution + MOLT4 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG
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![Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)](https://www.abcam.com/ps/products/249/ab249797/Images/ab249797-3-benefits-of-recombinant-antibodies.png)
Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (ab249797)
