Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP493Y] to Macrophage Inflammatory Protein 1 alpha / CCL3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies

  • Description

    Rabbit monoclonal [EP493Y] to Macrophage Inflammatory Protein 1 alpha / CCL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab181826 is the carrier-free version of ab46786. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab181826 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)
    Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)
    All lanes : Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786) at 1/2000 dilution

    Lane 1 : Human Macrophage Inflammatory Protein 1 alpha / CCL3 active protein
    Lane 2 : Human Macrophage Inflammatory protein 1 beta / CCL4 active protein
    Lane 3 : Human Macrophage Inflammatory active protein 4/CCL18
    Lane 4 : Human CCL3L1 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10, 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab46786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human CCL3 active protein contains aa27-92.

    Human CCL4 active protein contains aa24-92.

    Human CCL18 active protein contains aa21-89.

    Human CCL3L1 recombinant protein contain 2*(aa28-93) sequence with His-tag and GST-tag.

  • Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)
    Western blot - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)
    All lanes : Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) lysate
    Lane 2 : THP-1 lysate treated with 100 nM Phorbol-12-myristate-13-acetate (PMA) overnight, then treated with Lipopolysaccharides (LPS) at 100 ng/ml for 4 hour and then 1 µg/ml Brefeldin A was added for the last 3 hours.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa


    Exposure time: 3 minutes


    This data was developed using ab46786, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Macrophage inflammatory protein 1 alpha is induced in macrophages following exposure to bacterial lipopolysaccharide (LPS), PMID: 9848081.

  • Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)
    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EP493Y] - BSA and Azide free (ab181826)

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