Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13309] to PCQAP / MED15 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free
    See all PCQAP / MED15 primary antibodies

  • Description

    Rabbit monoclonal [EPR13309] to PCQAP / MED15 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250386 is the carrier-free version of ab181158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250386 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13309

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

    This data was developed using ab181158, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling PCQAP/MED15 with purified ab181158 at 1:250 dilution (1.28 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Flow Cytometry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Flow Cytometry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

    This data was developed using ab181158, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PCQAP/MED15 with purified ab181158 at 1:30 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (purified)

    Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 97 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181158, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (purified)

    Lane 1 : Mouse placenta lysates
    Lane 2 : Mouse spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa



    This data was developed using ab181158, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PCQAP/MED15 with Purified ab181158 at 1:100 dilution (3.2μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Flow Cytometry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    This data was developed using ab181158, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PCQAP / MED15 with unpurified ab181158 at 1/50 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Immunocytochemistry - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling PCQAP / MED15 with unpurified ab181158 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    This data was developed using ab181158, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PCQAP / MED15 with unpurified ab181158 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
  • Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Western blot - Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    All lanes : Anti-PCQAP / MED15 antibody [EPR13309] (ab181158) at 1/1000 dilution (unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 87 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    This data was developed using ab181158, the same antibody clone in a different buffer formulation.

     

  • Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)
    Anti-PCQAP / MED15 antibody [EPR13309] - BSA and Azide free (ab250386)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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