Anti-MED18 antibody (ab228887)
Key features and details
- Rabbit polyclonal to MED18
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED18 antibody
See all MED18 primary antibodies -
Description
Rabbit polyclonal to MED18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment within Human MED18 (internal sequence). The exact sequence is proprietary.
Database link: Q9BUE0 -
Positive control
- WB: A549 whole cell lysate. IHC: Cal27 xenograft. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MED18 antibody (ab228887)Anti-MED18 antibody (ab228887) at 1/1000 dilution + A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED18 antibody (ab228887)Paraffin-embedded Cal27 xenograft tissue stained for MED18 protein using ab228887 at 1/500 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-MED18 antibody (ab228887)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MED18 (green) using ab228887 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
