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Human NRG4 knockout HeLa cell lysate (ab258546)

Overview

  • Product name

    Human NRG4 knockout HeLa cell lysate

  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 2 bp deletion in exon3.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.

  • Sequence similarities

    Belongs to the neuregulin family.
    Contains 1 EGF-like domain.

  • Domain

    The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.

  • Post-translational
    modifications

    Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    Extensive glycosylation precedes the proteolytic cleavage.

  • Cellular localization

    Secreted and Cell membrane. Does not seem to be active.
  • Information by UniProt

  • Alternative names

    • DKFZp779N0541
    • DKFZp779N1944
    • Heregulin 4
    • HRG4
    • Neuregulin 4
    • Neuregulin-4
    • NRG-4
    • NRG4
    • NRG4_HUMAN
    • pro-neuregulin-4, membrane-bound isoform
    • Pro-NRG4
    see all

Properties

Images

  • Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)
    Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)

    Allele-1: 2 bp deletion in exon3

     

  • Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)
    Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)

    Allele-2: 1 bp deletion in exon3

     

  • Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)
    Sanger Sequencing - Human NRG4 knockout HeLa cell lysate (ab258546)

    Allele-3: 1 bp insertion in exon3

     

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