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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-RIP2 antibody [AF28D3] (ab75257)

Price and availability

308 236 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-RIP2 antibody [AF28D3] (ab75257)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [AF28D3] to RIP2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RIP2 antibody [AF28D3]
    See all RIP2 primary antibodies
  • Description

    Mouse monoclonal [AF28D3] to RIP2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human RIP2.

  • Positive control

    • WB: HeLa, 293T, Ramos, C6 and L929 cell lysates.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR3185124 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, HEPES
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    AF28D3
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Metabolism
    • Types of disease
    • Obesity
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Images

  • Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    All lanes : Anti-RIP2 antibody [AF28D3] (ab75257) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RIPK2 knockout HeLa cell lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 61 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control ab181602 observed at 37 kDa.

     ab75257 Anti-RIP2 antibody [AF28D3] was shown to specifically react with RIP2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264688 (knockout cell lysate ab258636) was used. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    All lanes : Anti-RIP2 antibody [AF28D3] (ab75257) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : RIPK2 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 61 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab75257 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and ab181602 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    Western blot - Anti-RIP2 antibody [AF28D3] (ab75257)
    All lanes : Anti-RIP2 antibody [AF28D3] (ab75257) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : L929 cell lysate

    Predicted band size: 61 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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