All lanes : Anti-PKN1 antibody (ab231038) at 1/500 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : SP2/0 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 104 kDa

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PKN1 (red) using ab231038 at 1/100 dilution in ICC/IF.

Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Formalin-fixed, paraffin-embedded human kidney tissue stained for PKN1 using ab231038 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human colon tissue stained for PKN1 using ab231038 at 1/100 dilution in immunohistochemical analysis.