Phospho-ERK2 (T185/Y187) and Total ERK2 (ab279794) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated ERK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-ERK2 and total ERK2. An anti-pan ERK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ERK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ERK2 (T185/Y187) antibody is added to detect phosphorylated ERK2. In the remaining wells, biotinylated anti-pan-ERK2 antibody is used to detect pan ERK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ERK2 (T185/Y187) or pan ERK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Neuroscience
• Neurotransmission
• Intracellular Signaling
• Kinases

• Signal Transduction
• Protein Phosphorylation
• Ser / Thr Kinases
• MAPK Pathway

• Stem Cells
• Signaling Pathways
• TGF beta
• Cytoplasmic