Anti-MED13/GCT antibody (ab76923)
Key features and details
- Rabbit polyclonal to MED13/GCT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED13/GCT antibody
See all MED13/GCT primary antibodies -
Description
Rabbit polyclonal to MED13/GCT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MED13/GCT.
Database link: NP_005112.2 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
This product was previously labelled as MED13
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MED13/GCT antibody (ab76923)All lanes : Anti-MED13/GCT antibody (ab76923) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 240 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-MED13/GCT antibody (ab76923)Detection of MED13/GCT by Western Blot of Immunprecipitate.
ab76923 at 1µg/ml staining MED13/GCT in HeLa whole cell lysate immunoprecipitated using ab76923 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
