Anti-Symplekin antibody (ab80274)
Key features and details
- Rabbit polyclonal to Symplekin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Symplekin antibody
See all Symplekin primary antibodies -
Description
Rabbit polyclonal to Symplekin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 350 and 400 of Human Symplekin (NP_004810.2).
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Positive control
- Whole cell lysate from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Symplekin antibody (ab80274)All lanes : Anti-Symplekin antibody (ab80274) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 141 kDa
Observed band size: 165 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-Symplekin antibody (ab80274)Detection of Symplekin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab80274 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.