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Anti-COMT antibody [EPR6490] (ab126618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6490] to COMT
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-COMT antibody [EPR6490]
    See all COMT primary antibodies

  • Description

    Rabbit monoclonal [EPR6490] to COMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human COMT. Human COMT is a soluble isoform.
    Database link: P21964

  • Positive control

    • WB: HEK-293T, HAP1, A431, MOLT4 and MCF7 cell lysates; Human fetal liver lysate. IHC-P: Human liver and kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : COMT knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 24-28 kDa
    why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)

    ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1 : Wild type HAP1 whole cell lysate at 40 µg
    Lane 2 : COMT knockout HAP1 whole cell lysate at 40 µg
    Lane 3 : A431 whole cell lysate at 20 µg
    Lane 4 : MCF7 whole cell lysate at 20 µg

    Predicted band size: 30 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MOLT4 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)

    ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-COMT antibody [EPR6490] (ab126618)
    Anti-COMT antibody [EPR6490] (ab126618)

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