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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)

Price and availability

321 638 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody [1D7D2A1]
    See all CDKN2A/p16INK4a primary antibodies
  • Description

    Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human CDKN2A/p16INK4a aa 1 to the C-terminus.
    Database link: P42771

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human CDKN2A protein; CDKN2A transfected HEK293 cells; HeLa, HepG2, HEK293 cell lysate. Flow Cytometry: HeLa cells. IHC-P: Human endometrial cancer and esophageal cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR286368-6 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1D7D2A1
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf

Images

  • Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution + recombinant human CDKN2A protein

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.

  • Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)

    Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN2A using ab201980 at 1/200 dilution (green) compared with a negative control (red).

  • Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : CDKN2A (AA: 1-156)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.

  • Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
    All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

    Predicted band size: 17 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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