Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
![Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243703-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-western-blot.jpg "Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)")
Key features and details
- Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [1D7D2A1]
See all CDKN2A/p16INK4a primary antibodies -
Description
Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human CDKN2A/p16INK4a aa 1 to the C-terminus.
Database link: P42771 -
Positive control
- WB: Recombinant human CDKN2A protein; CDKN2A transfected HEK293 cells; HeLa, HepG2, HEK293 cell lysate. Flow Cytometry: HeLa cells. IHC-P: Human endometrial cancer and esophageal cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR286368-6 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D7D2A1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution + recombinant human CDKN2A protein
Predicted band size: 17 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243699-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.
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![Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243700-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-flow-cytometry.jpg)
Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN2A using ab201980 at 1/200 dilution (green) compared with a negative control (red).
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![Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243702-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-western-blot.jpg)
Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution
Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CDKN2A (AA: 1-156)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 17 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243698-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.
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![Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)](https://www.abcam.com/ps/products/201/ab201980/Images/ab201980-243701-anti-cdkn2a-p16ink4a-antibody-1d7d2a1-western-blot.jpg)
Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 17 kDa
