Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 nmol/well
Overview
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Product name
Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.1 nmol/well -
Range
0.1 nmol/well - 10 nmol/well -
Assay time
0h 40m -
Product overview
Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
Visit our FAQs page for tips and troubleshooting. -
Notes
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests OxiRed Probe in DMSO Red 1 x 200µl PC Assay Buffer WM 1 x 25ml PC Development Mix (Lyophilised) Green 1 vial PC Hydrolysis Enzyme (lyophilized) Purple 1 vial PC Standard (10 µmol) (Lyophilised) Yellow 1 vial -
Research areas
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Relevance
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. -
Alternative names
- PC
Images
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Functional Studies - Phosphatidylcholine Assay Kit (ab83377)
Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Phosphatidylcholine Assay Kit (ab83377)Phosphatidycholine levels colorimetrically measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Phosphatidylcholine Assay Kit (ab83377)Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Phosphatidylcholine Assay Kit (ab83377)Examples of Colorimetric and Fluorometric Phosphatidylcholine standard curves using ab83377.