Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab270171 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10µg/mL human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab270171 or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 µl at 0.2µg/mL (1/2500)) for 30 min on ice. The cells were simultaneously stained with NCAM.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live lymphocytes.