Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)
![Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)](https://www.abcam.com/ps/products/276/ab276046/Images/ab276046-1-AntiOLFM4-antibody-12-immunofluorescence-A431-cells.jpg "Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)")
Key features and details
- Mouse monoclonal [12] to OLFM4 - BSA and Azide free
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-OLFM4 antibody [12] - BSA and Azide free
See all OLFM4 primary antibodies -
Description
Mouse monoclonal [12] to OLFM4 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
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Immunogen
Recombinant full length protein corresponding to Human OLFM4 aa 1-510. NP_006409.3
Database link: Q6UX06 -
Positive control
- ICC: A431 cells. Flow cyt: DU145 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
12 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-OLFM4 antibody [12] (ab276046)
Immunofluorescence staining of OLFM4 in A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276046 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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![Flow Cytometry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)](https://www.abcam.com/ps/products/276/ab276046/Images/ab276046-412453-ab2760462AntiOLFM4antibody12flowcytDU145cells.jpg)
Flow Cytometry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)Flow cytometric analysis of OLFM4 expression in DU145 cells. The cells were stained with ab276046 at 1/25 dilution, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.