Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PAIP1 antibody [EPR13259] (ab181359)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13259] to PAIP1
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-PAIP1 antibody [EPR13259]
See all PAIP1 primary antibodies
Description

Rabbit monoclonal [EPR13259] to PAIP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAIP1 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H074
Positive control
- HepG2, HeLa, and 293T cell lysates, Human gastric carcinoma tissue, Human uterus tissue, HeLa cells, permeabilized 293 cells, immunoprecipitation pellet from HeLa whole cell lysate (ab150035)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13259
Isotype

IgG
Research areas

Western blot - Anti-PAIP1 antibody [EPR13259] (ab181359)

All lanes : Anti-PAIP1 antibody [EPR13259] (ab181359) at 1/1000 dilution

Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 54 kDa
Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (ab181359)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab181359 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Flow Cytometry - Anti-PAIP1 antibody [EPR13259] (ab181359)

Flow cytometric analysis of permeabilized 293 cells using ab181359 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (ab181359)

Immunofluorescence analysis of HeLa cells labeling PAIP1 with ab181359 at 1/50 dilution (green). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling PAIP1 with ab181359 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling PAIP1 with ab181359 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PAIP1 antibody [EPR13259] (ab181359)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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