Anti-SV40 T-antigen antibody [PAb416] (ab16879)
Key features and details
- Mouse monoclonal [PAb416] to SV40 T-antigen
- Suitable for: ICC, ICC/IF, WB, IP, IHC-P
- Isotype: IgG2a
Overview
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Product name
Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies -
Description
Mouse monoclonal [PAb416] to SV40 T-antigen -
Host species
Mouse -
Tested applications
Suitable for: ICC, ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
SV40 infected cells. -
Immunogen
SV40 transformed mouse cells
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Epitope
Within amino acids 83-128 of the large T-antigen. -
Positive control
- SV80 or SV-T2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PAb416 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
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ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.