Human ADAMTS13 Antibody Pair - BSA and Azide free (ab244105)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human ADAMTS13 Antibody Pair - BSA and Azide free
See all ADAMTS13 kits -
Assay type
ELISA set -
Range
0.125 ng/ml - 10 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human ADAMTS13. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234559), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human ADAMTS13 Capture Antibody (unconjugated) 1 x 100µg Human ADAMTS13 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Cleaves the vWF multimers in plasma into smaller forms. -
Tissue specificity
Plasma. Expressed primarily in liver. -
Involvement in disease
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. -
Sequence similarities
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains. -
Domain
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction. -
Post-translational
modificationsMay contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 13
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 13
see all -
Database links
- Entrez Gene: 11093 Human
- Omim: 604134 Human
- SwissProt: Q76LX8 Human
- Unigene: 131433 Human
Images
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Sandwich ELISA - Human ADAMTS13 Antibody Pair - BSA and Azide free (ab244105)To learn more about the advantages of recombinant antibodies see here.
