Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dopamine Transporter antibody [EPR19695] (ab184451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19695] to Dopamine Transporter
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Dopamine Transporter antibody [EPR19695]
    See all Dopamine Transporter primary antibodies

  • Description

    Rabbit monoclonal [EPR19695] to Dopamine Transporter

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Unboiled Mouse and rat striatum lysates. IHC-P: Mouse and rat striatum tissues. IHC-Fr: Mouse brain (Coronal section) and rat brain (sagittal section) tissues. IP: Mouse striatum whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19695

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Western blot - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    All lanes : Anti-Dopamine Transporter antibody [EPR19695] (ab184451) at 1/1000 dilution

    Lane 1 : Mouse striatum lysate boiled
    Lane 2 : Mouse striatum lysate unboiled
    Lane 3 : Rat striatum lysate boiled
    Lane 4 : Rat striatum lysate unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 69 kDa



    We recommend not to boil the samples after lysis to get desired WB results

  • Western blot - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Western blot - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    All lanes : Anti-Dopamine Transporter antibody [EPR19695] (ab184451) at 1/1000 dilution

    Lane 1 : Mouse striatum lysate
    Lane 2 : Rat striatum lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat liver lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa
    Observed band size: 70-85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra (PMID:1765147), so non-related tissues and cells were chosen as negative controls; and DAT is a glycoprotein with molecular weight of 70-85KD (PMID:25356398, PMID: 20643191).

  • Immunohistochemistry (Frozen sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Frozen sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Dopamine Transporter with ab184451 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
    The result showed cytoplasmic staining on rat striatum.
    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Dopamine Transporter with ab184451 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
    Membrane and cytoplasm staining on mouse striatum is observed.
    Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Dopamine Transporter with ab184451 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
    Counter stained with Hematoxylin.
     
    Negative control: Negative staining on mouse liver.
    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Dopamine Transporter with ab184451 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
    Cytoplasm staining on rat striatum is observed.
    Counter stained with Hematoxylin. 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Dopamine Transporter with ab184451 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
    Counter stained with Hematoxylin.
     
    Negative control: Negative staining on rat spleen.
    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Frozen sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemistry (Frozen sections) - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Dopamine Transporter with ab184451 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
    The result showed cytoplasmic staining on mouse striatum but negative on lateral ventricle (LA).
    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Immunoprecipitation - Anti-Dopamine Transporter antibody [EPR19695] (ab184451)

    Dopamine Transporter was immunoprecipitated from 0.35mg of Mouse striatum whole cell lysate with ab184451 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab184451 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse striatum whole cell lysate, 10µg (Input).

    Lane 2: ab184451 IP in Mouse striatum whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184451 in Mouse striatum whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Dopamine Transporter antibody [EPR19695] (ab184451)
    Anti-Dopamine Transporter antibody [EPR19695] (ab184451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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