Anti-Dopamine Transporter antibody (ab130140)
Key features and details
- Goat polyclonal to Dopamine Transporter
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Dopamine Transporter antibody
See all Dopamine Transporter primary antibodies -
Description
Goat polyclonal to Dopamine Transporter -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide:
C-QLTSSTLTNPRQSP
(with a Cysteine residue linker), corresponding to internal sequence amino acids 41-54 of Human Dopamine Transporter (NP_001035.1; Q01959). -
Positive control
- Human Brain (Substantia nigra) lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab130140 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Dopamine Transporter antibody (ab130140)Anti-Dopamine Transporter antibody (ab130140) at 1 µg/ml + Human Substantia nigra lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28):7040-6. PMID: 18614672).
