Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)
![Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)](https://www.abcam.com/ps/products/128/ab128848/Images/ab128848-194842-anti-dopamine-transporter-antibody-6v-23-23-western-blot.jpg "Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)")
Key features and details
- Mouse monoclonal [6V-23-23] to Dopamine Transporter
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Dopamine Transporter antibody [6V-23-23]
See all Dopamine Transporter primary antibodies -
Description
Mouse monoclonal [6V-23-23] to Dopamine Transporter -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide corresponding to Rat Dopamine Transporter (N terminal).
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Positive control
- In western blot, this antibody gave a positive signal in mouse and rat forebrain tissue lysates, and in rat brain striatal lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6V-23-23 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)All lanes : Anti-Dopamine Transporter antibody [6V-23-23] (ab128848) at 5 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Striatal Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128848 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Rat cerebellum (lane 3) was included as a negative control. This tissue does not express dopamine transporter protein.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)](https://www.abcam.com/ps/products/128/ab128848/Images/ab128848-264047-anti-dopamine-transporter-antibody-6v-23-23-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [6V-23-23] (ab128848) Carl Hobbs (Kings College, London, United Kingdomab128848 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
