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Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22694-148] to SV40 T-antigen
  • Suitable for: ICC/IF, IP, Flow Cyt, WB

Overview

  • Product name

    Anti-SV40 T-antigen antibody [EPR22694-148]
    See all SV40 T-antigen primary antibodies

  • Description

    Rabbit monoclonal [EPR22694-148] to SV40 T-antigen

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details

  • Species reactivity


    Predicted to work with: a wide range of other species, A wide range of other invertebrates

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T (transfected) cell lysate. ICC/IF: HEK-293T cells. Flow: HEK-293T (SV40 transfected). IP: HEK-239T whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    Western blot - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    All lanes : Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/1000 dilution

    Lane 1 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Negative control: HEK-293 (PMID: 15175262).

    Exposure time: 10 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.

    Negative control: HEK-293(PMID: 15175262).

  • Flow Cytometry - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    Flow Cytometry - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with a Rabbit monoclonal IgG isotype control (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    Negative control: HEK-293.

  • Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

    SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 µg (input).
    Lane 2: ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)
    Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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