Anti-SUV420h1 antibody (ab118659)
Key features and details
- Rabbit polyclonal to SUV420h1
- Suitable for: ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SUV420h1 antibody
See all SUV420h1 primary antibodies -
Description
Rabbit polyclonal to SUV420h1 -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Zebrafish
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Immunogen
Synthetic peptide corresponding to a region within C terminal amino acids 271-320 (FINHDCRPNC KFVSTGRDTA CVKALRDIEP GEEISCYYGD GFFGENNEFC) of Mouse SUV420h1 (NP_001161356).
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Positive control
- WB: Mouse Thymus lysate. ChIP: HCT116 colon cancer cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SUV420h1 antibody (ab118659)Anti-SUV420h1 antibody (ab118659) at 1 µg/ml + Mouse Thymus lysate at 10 µg
Predicted band size: 38 kDa
Gel concentration: 12% -
ChIP - Anti-SUV420h1 antibody (ab118659)Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
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ChIP - Anti-SUV420h1 antibody (ab118659)ChIP analysis using ab118659 (0.5µg/mL) binding SUV420h1 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.