Anti-PPP1CB antibody (ab154600)
Key features and details
- Rabbit polyclonal to PPP1CB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPP1CB antibody
See all PPP1CB primary antibodies -
Description
Rabbit polyclonal to PPP1CB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human PPP1CB aa 1-274.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysate. Mouse brain whole cell lysate. HeLa cells.
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General notes
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP1CB antibody (ab154600)All lanes : Anti-PPP1CB antibody (ab154600) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
7.5% SDS PAGE -
Western blot - Anti-PPP1CB antibody (ab154600)Anti-PPP1CB antibody (ab154600) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 37 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CB antibody (ab154600)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PPP1CB with ab154600 at 1/500 dilution. Lower panel is co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (ab154600)Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling PPP1CB with ab154600 at 1/100 dilution.
