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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-PPP1CB antibody (ab245656)

Anti-PPP1CB antibody (ab245656)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PPP1CB
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PPP1CB antibody
    See all PPP1CB primary antibodies
  • Description

    Rabbit polyclonal to PPP1CB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human PPP1CB aa 280-327. The exact sequence is proprietary. NP_996759.1
    Database link: P62140

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245656 was affinity purified using an epitope specific to Protein Phosphatase 1 beta immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (ab245656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (ab245656)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Protein Phosphatase 1 beta with ab245656 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-PPP1CB antibody (ab245656)
    Western blot - Anti-PPP1CB antibody (ab245656)
    All lanes : Anti-PPP1CB antibody (ab245656) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 37 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-PPP1CB antibody (ab245656)
    Immunoprecipitation - Anti-PPP1CB antibody (ab245656)

    Protein Phosphatase 1 beta was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245656 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245656 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PPP1CB antibody (ab245656)

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Applications: ICC/IF, IHC-P, WB

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