Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric) (ab273302)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Male Serum
Overview
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Product name
Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric)
See all Prostate Specific Antigen kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Male Serum -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric) (ab273302) utilizes the ability of an active PSA to cleave a synthetic AMC based peptide substrate to release a free fluorophore. The released AMC can be easily quantified using a fluorescent microplate reader.
This assay kit is simple and can be used in a highthroughput format.
This assay can detect as low as 1 ng of purified active PSA.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMC-Standard (1 mM) 1 x 100µl PSA Assay Buffer 1 x 25ml PSA Dilution Buffer 1 x 1.8ml PSA Positive control 1 x 10µl PSA Substrate (10 mM) 1 x 400µl -
Research areas
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Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- antigen, prostate-specific
- APS
- Gamma seminoprotein
see all
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataPSA activity: In Positive Control and in 100x diluted (6 µl) seminal fluid.
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Example dataKinetic activity curves using different amounts of PSA Positive Control in the assay.
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Example dataPSA in seminal fluid: Amount of PSA in ng in 2 to 10 µl of 100x diluted seminal fluid samples.